Osteogenic Differentiation of Mesenchymal Stem Cells Via Osteoblast- Imprinted Substrate: In Vitro and In Vivo Evaluation in Rat Model

Authors

  • Hossein Gholami Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Mohammad Mehdi Dehghan Department of Surgery & Radiology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran.
  • Saeed Farzad Mohajeri department of radiology and surgery, faculty of veterinary medicine, university of Tehran,Tehran, Iran.
Abstract:

BACKGROUND: Stem cells have great effects in clinical cell-based therapy. Accordingly, controlling the behavior and directing the fate of stem cells cultured in the laboratory is an important issue. OBJECTIVES: The aim of this study was to evaluate osteogenic properties of adipose derived mesenchymal stem cells (ADSCs) which differentiated toward osteogenic linage by osteoblast-imprinted substrate. METHODS: Rat ADSCs seeded on osteoblast-imprinted substrates, alkaline phosphatase (ALP) were measured in cellular supernatant of days 7 and 14. Alizarin Red staining of mineral matrix production in day 17 was performed. In order for in vivo evaluation, after seeding differentiated cells on a simple collagen scaffold, samples were implanted in an 8mm critical size calvarial defect. After 4 weeks defect site was harvested and prepared for histopathological examination. RESULTS: ALP in both time points was significantly more than in undifferentiated ADSCs. (p

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Journal title

volume 13  issue 3

pages  260- 269

publication date 2019-09-01

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